140 Effectiveness of Washing Procedures for Removing Brucella Abortus from In Vivo-Derived Wood Bison Embryos

A study on wood bison embryos exposed to Brucella abortus demonstrates that a 10-step washing procedure can effectively remove the pathogen. The research found that 89% of embryos were cleared of Brucella using a wash medium without antibiotics, while 100% were cleared when antibiotics (penicillin and streptomycin) were added. Brucella was undetectable in wash media after the third wash in both groups. This validates embryo washing as a reliable technique for producing disease-free wood bison embryos, crucial for conserving genetic diversity in Wood Buffalo National Park, where endemic brucellosis threatens wild herds.

Palomino, J. M., Cervantes, M. P., Mastromonaco, G., Mapletoft, R. J., Allan, B., & Adams, G. P. (2014). 140 EFFECTIVENESS OF WASHING PROCEDURES FOR REMOVING BRUCELLA ABORTUS FROM IN VIVO-DERIVED WOOD BISON EMBRYOS. Reproduction, Fertility and Development, 27(1), 162. https://doi.org/10.1071/rdv27n1ab140